In this video, Prof. Ron M.A. Heeren, Distinguished Professor of Molecular Imaging, Limburg chair at Maastricht University, Netherlands, and Director of The Maastricht MultiModal Molecular Imaging institute, M4i, describes the value of imaging mass spectrometry. Heeren discusses how this newly developed technology could add a new layer of information within pathology by enabling very high spatial resolution, allowing molecular analysis within a single cell. This can be combined with targeted proteomics to gain lipidome and proteome images, in single cell resolution, and applied in the context of disease. Heeren discusses what he hopes for the future of imaging mass spectrometry, and explains why, in this age of interdisciplinary collaborations, you need to bring the right people together to tackle these very complex questions.